| CD 1 | |
| 1 | | I Am a Man of Constant Sorrow - - - The Stanley Brothers |
| 2 | | John Henry - - - The Bailey Brothers |
| 3 | | Earl's Breakdown - - - Flatt and Scruggs |
| 4 | | In the Pines - - - Bill Monroe |
| 5 | | There's Another Baby Waiting for Me Down the Line - - - Reno & Smiley |
| 6 | | Hold the Woodpile Down - - - Uncle Dave Macon |
| 7 | | Bluegrass Breakdown - - - Bill Monroe |
| 8 | | Going Back to the Blue Ridge Mountains - - - The Delmore Brothers |
| 9 | | Hawkins Rag - - - Gid Tanner's Skillet Lickers |
| 10 | | Molly & Tenbrooks - - - The Stanley Brothers |
| 11 | | Dim Lights Thick Smoke - - - Flatt and Scruggs |
| 12 | | Mule Skinner Blues - - - Bill Monroe |
| 13 | | Are You from Dixie? - - - The Blue Sky Boys |
| 14 | | Tennessee Cut-up Breakdown - - - Reno & Smiley |
| 15 | | The Little Glass of Wine - - - The Stanley Brothers |
| 16 | | Beautiful Brown Eyes - - - The Bailey Brothers |
| 17 | | Blue Yodel No. 4 - - - Bill Monroe |
| 18 | | Footprints in the Snow - - - Cliff Carlisle |
| 19 | | Foggy Mountain Special - - - Flatt and Scruggs |
| 20 | | Sally Goodin - - - Ernest V. Stoneman & His Dixie Mountaineers |
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| CD 2 | |
| 1 | | Cotton Eyed Joe - - - The Stanley Brothers |
| 2 | | New Mule Skinner Blues - - - Bill Monroe |
| 3 | | Happy Valley Special - - - The Bailey Brothers |
| 4 | | Feudin' Banjos - - - Don Reno & Arthur Smith |
| 5 | | I'm Going to Make Heaven My Home - - - Flatt and Scruggs |
| 6 | | In the Hills of Roane County - - - The Blue Sky Boys |
| 7 | | Go Along Mule - - - Uncle Dave Macon |
| 8 | | Orange Blossom Special - - - The Rouse Brothers |
| 9 | | She's Just a Cute Thing - - - Jimmy Martin & Osborne Brothers |
| 10 | | Air Mail Special - - - Jim & Jesse |
| 11 | | Don't Get Above Your Raisin' - - - Flatt and Scruggs |
| 12 | | I'm Going Back to Old Kentucky - - - Bill Monroe |
| 13 | | Banjo Pickin' Girl - - - Coon Creek Girls |
| 14 | | Mountain Dew - - - Grandpa Jones |
| 15 | | I'm Gone, Long Gone - - - Reno & Smiley |
| 16 | | The Great Speckled Bird - - - Charlie Monroe's Boys |
| 17 | | Roll in My Sweet Baby's Arms - - - Bill & Charlie Monroe |
| 18 | | Doin' My Time - - - Jimmie Skinner |
| 19 | | Poor Ellen Smith - - - Molly O'Day |
| 20 | | Will the Circle Be Unbroken - - - Brownsferry Four |
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| CD 3 | |
| 1 | | Blue Moon of Kentucky - - - Bill Monroe |
| 2 | | Cabin Home in Carolina - - - The Armstrong Twins |
| 3 | | Nobody's Love Is Like Mine - - - The Stanley Brothers |
| 4 | | Hop Light Ladies - - - Ernest V. Stoneman & His Dixie Mountaineers |
| 5 | | My Lonely Heart - - - Jimmy Martin |
| 6 | | Buddy Won't You Roll Down the Line - - - Uncle Dave Macon |
| 7 | | Uncle Penn - - - Bill Monroe |
| 8 | | Heart's Hall of Fame - - - The Bailey Brothers |
| 9 | | Little Birdie - - - Wade Mainer |
| 10 | | The Fields Have Turned Brown - - - The Stanley Brothers |
| 11 | | Run Mountain - - - J.E. Mainer |
| 12 | | I'm Lonesome Without You - - - The Delmore Brothers |
| 13 | | Get in Line Brothers - - - Flatt and Scruggs |
| 14 | | Banjo Twist - - - The McCormic Brothers |
| 15 | | Ruby Are You Mad at Your Man? - - - The Osborne Brothers |
| 16 | | I'm the Talk of the Town - - - Reno & Smiley |
| 17 | | I'll Wash Your Love from My Heart - - - Jim & Jesse |
| 18 | | Y'all Come - - - Bill Monroe |
| 19 | | When I Safely Reach That Other Shore - - - Tommy Magness |
| 20 | | Foggy Mountain Breakdown - - - Flatt and Scruggs |